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After the cells reached 75% confluence, they were transfected with 1nM pre-miRNA-precursor miR-320d (Qiagen, Hilden, Germany) or 1nM mimic control (Qiagen) using RNAimax (Invitrogen) in Gibco Opti-MEM (ThermoFisher) for 4h

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Pregnant women who smoke or vape are at greater risk for complications such as preterm labor, premature birth and lower birth weight babies

Removing smoke smell requires thorough cleaning, as well as addressing the underlying causes of the odor, not just masking it